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Home » GATE Study Material » Pharmaceutical Science » Micro Biology » The Prokaryotes: Domains Bacteria and Archaea

The Prokaryotes: Domains Bacteria and Archaea

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The Prokaryotes: Domains Bacteria and Archaea

The Prokaryotes: Domains Bacteria and Archaea

Three basic prokaryote shapes: cocci, spiral and bacilli.

  • Cocci are roughly spherical to oval shaped.

  • Bacilli are shaped like a prolate ellipsoid, but can appear filamentous or spindly.

  • Spiral shaped prokaryotes.

  • There are some variations of these shapes but these are predominant.



Cell arrangements:

  • The final arrangement that the cells take depends on plane in which the cell divides. In binary fission the planes in which the cell divide determine arrangement.

  • Diplococci are cocci pairs that remain attached create long chains called streptococci.

  • Cocci that divide in two planes and stay attached form tetrads.

  • Division in three planes form cuboidal packets called sarcinae. 

  • Clusters of cocci are called staphylococci and they resemble a bunches of grapes.

  • Bacilli have less variability in arrangement because they divide perpendicular to the long axis. 

  • The diphtheriae bacteria divide by snapping and they form a V shape or side by side stacking called palisade. 

  • In hostile environments some bacteria can form Endospores.   Endospores are not the same as reproductive spores. 

Steps of endospore formation:

  • DNA is replicated.

  • DNA aligns with the long axis of the cell.

  • The cytoplasmic membrane invaginates forming the forespore.

  • The cell membrane continues to grow and the forespore is enveloped within a second membrane.  The DNA of the vegetative cell disintegrates.

  • A spore coat is formed around the endospore.

  • The endospore matures by the completion of the spore coat.

  • The endospore is released.

Extremophiles

  • Microbes that need extreme conditions of: temperature, pH, salinity or atmosphere to survive.

DNA Facts

  • The DNA of a cell is made up of guanine, cytosine, thymine and adenine.  Guanine pairs with cytosine in DNA.  The G+C ratio refers to the amount, in percent, of guanine and cytosine in a cells DNA.  G+C ratio below 50% are considered log G+C bacteria.  Above 50% G+C ratio is considered high.  Gram-positive bacteria with low G+C ratios have similar 16s rRNA sequences and those with high G+C have rRNA sequences in common.

  • Taxonomists have used the G+C ratio as a criteria for placing bacteria in phyla.

  • Gram positive high G+C bacteria include bacteria that have rod shaped cells and filamentous bacteria.  The latter look like fungi in how they grow and reproduce and sporulate.



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