Plotting 1/velocity against [inhibitor] (NB not
1/[inhibitor]) often yields linear graphs from which the Ki can be
estimated.
IC50
The IC50 can be estimated from a plot of velocity
against log[inhibitor]. The IC50 is the inhibitor concentration where
the velocity is cut by half. At low substrate concentrations, Ki~IC50
Active site models
This is a representation of angiotensin I bound to
the active centre.
This is a representation of captopril bound to the
active centre.
